System and method useful for sarcomere imaging via objective-based microscopy

US10499797B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Gabriel Sanchez · Stanford, US
• Scott L. Delp · Stanford, US
• Mark J. Schnitzer · Summit, US
• Michael E. Llewellyn · Palo Alto, US

Key dates

• Filing date: Nov 18, 2014
• Grant date: Dec 10, 2019
• Expiry date: Nov 18, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/6486
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Biological tissue such as skeletal and cardiac muscle can be imaged by using an objective-based probe in the tissue and scanning at a sufficiently fast rate to mitigate motion artifacts due to physiological motion. According to one example embodiment, such a probe is part of a system that is capable of reverse-direction high-resolution imaging without needing to stain or otherwise introduce a foreign element used to generate or otherwise increase the sensed light. The probe can include a light generator for generating light pulses that are directed towards structures located within the thick tissue. The system can additionally include aspects that lessen adverse image-quality degradation. Further, the system can additionally be constructed as a hand-held device.