Allophycocyanin alpha-subunit evolved labeling proteins (smURFPs)

US10501502B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Erik Rodriguez · Houston, US
• John Lin · Cabin John, US
• Roger Y. Tsien · Village of La Jolla, US
• Richard Ting · New York, US
• Geraldine Tran · Village of La Jolla, US

Key dates

• Filing date: Nov 22, 2017
• Grant date: Dec 10, 2019
• Expiry date: Nov 22, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/195
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides evolved red-shifted smURFPs. In some embodiments, the smURFPs are characterized by a) increased Fluorescence of smURFP as compared to infrared FPs IFP1.4 and iRFP713; b) expressing efficiently with minimal toxicity; c) does not require a lyase to covalently attach its chromophore, wherein the chromophore is biliverdin; d) exhibit a wavelengths longer than attainable with jellyfish- or coral-derived FPs using smURFP and IFP2.0; e) allows for functional fusion to hCdt1(30/120) as compared to jellyfish- or coral-derived FPs mAG, eGFP, and mRFP1 which are nonfunctional; and f) allows for deep tissue imaging.