Method of preparing functional peptides from germinated beans

US10501509B2 · kind B2 · utility

Assignee

• ZHEJIANG ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

• Wei Wang · Beijing, CN
• Nan Wang · Shanghai, CN
• Yu Zhang · Sichuan, CN
• Junhong Wang · Shanghai, CN
• Zuoyi Zhu · Nanhu, CN
• Xue Li · Beijing, CN
• Jiahong Zhu · Zhejiang, CN

Key dates

• Filing date: Jan 11, 2017
• Grant date: Dec 10, 2019
• Expiry date: Nov 13, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/06
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention discloses a method of preparing a functional peptide from a germinated bean, including the steps of: germinating a bean until a sprout length is about 0.5-1.0 cm; adding water to the obtained germinated bean, and then blending and crushing the germinated bean; adjusting the obtained slurry to a pH value between 6.5-8.0, and then adding neutral protease, papain and compound flavor protease to perform enzymatic reaction under stirring condition, wherein an enzymatic reaction solution is obtained including the functional peptide; subjecting the enzymatic reaction solution to ultrafiltration using an ultrafiltration membrane; keeping the ultrafiltrated enzymatic reaction solution at a temperature between 70-80° C. or using ozonated water at a concentration between 20-25 mg/L thereby avoiding decomposition by microorganisms; and performing spray drying to obtain the functional peptide.