Cell-free synthesis of DNA by strand displacement
US10501782B1 · kind B1 · utility
2Cited by
3References
31Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 21, 2015 |
| Grant date | Dec 10, 2019 |
| Priority date | — |
| Expiry date | Aug 21, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.