Patent · US Active

Nucleic acid detection and quantification

US10501783B2 · kind B2 · utility

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6Claims

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Assignee

QIAGEN GmbH · DE

Inventors

Nan Fang · Lo Wu, CN
Andreas Missel · Mülheim an der Ruhr, DE
Katja Heitz · Hilden, DE
Holger Wedler · Hilden, DE

Key dates

Filing date	Dec 9, 2014
Grant date	Dec 10, 2019
Priority date	—
Expiry date	Sep 30, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/686
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to methods and uses for the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules in a sample during quantitative real-time polymerase chain reaction (qPCR) amplification. According to the invention, an intercalating dye recognizing double-stranded DNA molecules with higher affinity than single-stranded DNA molecules and a fluorophore-labeled oligonucleotide-probe being sequence specific for a target nucleic acid molecule are simultaneously employed, thus enabling quantification a specific target and total amount of a mixed nucleic acid population, and enabling assessing the cause of suboptimal PCR performance.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.