Nucleic acid encoding reactions

US10501786B2 · kind B2 · utility

Assignee

• FLUIDIGM CORPORATION · US

Inventors

• Megan Anderson · Vienna, US
• Peilin Chen · Richmond, US
• Brian Fowler · San Mateo, US
• Robert C. Jones · Los Altos, US
• Fiona Kaper · San Diego, US
• Ronald Lebofsky · Mississauga, CA
• Andrew Paul May · South San Francisco, US

Key dates

• Filing date: May 28, 2015
• Grant date: Dec 10, 2019
• Expiry date: May 28, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.