Microarray based multiplex pathogen analysis and uses thereof
US10501814B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 11, 2018 |
| Grant date | Dec 10, 2019 |
| Priority date | — |
| Expiry date | Oct 11, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.