Polynucleotide probe design

US10504612B2 · kind B2 · utility

Assignee

• Emerald Therapeutics, Inc. · US

Inventors

• Brian M. Frezza · Redwood City, US
• Bradley M. Bond · Palo Alto, US
• Collin Melton · San Francisco, US
• Catherine L. Hofler · San Francisco, US
• Daniel J. Kleinbaum · Redwood City, US

Key dates

• Filing date: Mar 13, 2013
• Grant date: Dec 10, 2019
• Expiry date: Mar 13, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B35/00
• WIPO field: Computer technology
• WIPO sector: Electrical engineering

Abstract

An approach to designing a polynucleotide probe to hybridize selectively to a target polynucleotide sequence involves calculating the final concentration of the intended binding product between a candidate probe and the target sequence. The calculation takes into consideration the binding reaction between the candidate probe and the target fragment on the target sequence, as well as various other binding reactions, involving either the probe or the target fragment, that interfere with the intended binding reaction. In contrast to the conventional technology, which attempts to determine the entire structure of the target polynucleotide, this approach only needs to determine the binding dynamics that impact on the intended probe-target fragment binding. The approach does not require determination of the structure of the involved sequences.