Separation matrix

US10513537B2 · kind B2 · utility

Assignee

• GE Healthcare BioProcess R&D AB · SE

Inventors

• Gustav Rodrigo · Uppsala, SE
• Tomas Bjorkman · Uppsala, SE
• Mats Ander · Uppsala, SE
• Jesper Ulf Hansson · Uppsala, SE

Key dates

• Filing date: May 10, 2017
• Grant date: Dec 24, 2019
• Expiry date: May 10, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K17/10
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.