Method for differentiating pluripotent mammalian stem cells into a population of hepatic cells in a microchamber
US10513685B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 23, 2011 |
| Grant date | Dec 24, 2019 |
| Priority date | — |
| Expiry date | Mar 23, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2535/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.