Cartridge for a magnetic flow cytometer, a magnetic flow cytometer, and method for analysing a sample with such a cartridge

US10520419B2 · kind B2 · utility

Assignee

• EarlyBio GmbH · DE

Inventors

• Oliver Hayden · Moosburg an der Isar, DE
• Michael Johannes Helou · Regensburg, DE
• Mathias Reisbeck · Regensburg, DE
• Lukas Richter · Hirschaid, DE

Key dates

• Filing date: May 20, 2014
• Grant date: Dec 31, 2019
• Expiry date: May 20, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1024
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a cartridge (1) for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet (2) for injecting a sample (15) into the cartridge (1), a blister (3) for a buffer solution (21) with magnetic markers to mark pregiven particles (16, 16′) of the sample (15), an outlet, and a fluid channel (9), the fluid channel (9) comprising a first part that connects the inlet (2) with the blister (3) and a second part that connects the first part with the outlet, wherein the second part of the fluid channel (9) comprises an enrichment zone (5) with mechanical guiding structures to focus marked particles (16, 16′) of the sample (15) in a predetermined subsection of the fluid channel (9) and a measuring zone (6) between the enrichment zone (5) and the outlet, the measuring zone (6) comprising a magnetic field sensor (14) in the predetermined subsection of the fluid channel (9) in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample.