Patent · US Active

Lab color space silver and red in situ hybridization based techniques for detecting genes in tissue samples

US10521644B2 · kind B2 · utility

1Cited by
6References
7Claims
0Family size

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Key dates

Filing dateJan 29, 2013
Grant dateDec 31, 2019
Priority date
Expiry dateSep 14, 2036

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06T2207/30024
  • WIPO fieldComputer technology
  • WIPO sectorElectrical engineering

Abstract

A computer-based specimen analyzer (10) is configured to detect a level of expression of genes in a cell sample by detecting dots that represent differently stained genes and chromosomes in a cell. The color of the stained genes and the chromosomes is enhanced and filtered to produce a dot mask that defines areas in the image that are genes, chromosomes, or non-genetic material. Metrics are determined for the dots and/or pixels in the image of the cell in areas corresponding to the dots. The metrics are fed to a classifier that separates genes from chromosomes. The results of the classifier are counted to estimate the expression level of genes in the tissue samples.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.