Droplet sequencing device
US10525459B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 6, 2016 |
| Grant date | Jan 7, 2020 |
| Priority date | — |
| Expiry date | Aug 26, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1041
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for sequencing a nucleic acid by printing droplets at least some of which contain single nucleotides derived from the nucleic acid is provided. It is characterised by comprising; •a planar substrate having a face with droplet-receiving locations arranged in at least one track parallel to a first axis defining the substrate; ⋅a printer head comprising a plurality of droplet-dispensing nozzle groups juxtaposed above the droplet-receiving locations so that each nozzle group can in turn dispense a droplet into the droplet-receiving locations; •a means for stepping the printer head along the first axis relative to the droplet-receiving locations; ⋅a nucleotide-generating site upstream of the printer head by means of which an ordered stream of single nucleotides is created from the nucleic acid and delivered to a first droplet-dispensing nozzle group; •at least one source of incident electromagnetic radiation adapted to illuminate the droplet-receiving locations and ⋅at least one photodetector to detect adapted to detect fluorescence radiation emitted by each droplet-receiving location after illumination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.