Compounds and methods for CRISPR/Cas-based genome editing by homologous recombination
US10526590B2 · kind B2 · utility
3Cited by
5References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 30, 2016 |
| Grant date | Jan 7, 2020 |
| Priority date | — |
| Expiry date | Aug 11, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3519
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.