Method of improving characteristics of proteins
US10526597B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 16, 2016 |
| Grant date | Jan 7, 2020 |
| Priority date | — |
| Expiry date | Dec 12, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides efficient methods for combining single-substitution libraries of nucleic acids that span and encode proteins of interest and for selecting resultant mutant proteins after expression which have improved properties or characteristics. Specifically, the methods comprising synthesizing a single substitution library for each of a plurality of domains of a protein; expressing separately each member of each single substitution library as a pre-candidate protein; selecting members of each single substitution library which encode pre-candidate proteins which exhibit an improvement in the one or more predetermined characteristics to form a selected library; shuffling members or the selected libraries in a PCR to produce a combinatorial shuffled library; expressing members of the shuffled library as candidate proteins; and selecting mutant proteins which have improved properties or characteristics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.