Preparation method for (R)-3-hydroxyl-5-hexenoate

US10526622B2 · kind B2 · utility

Assignee

• FUDAN UNIVERSITY · CN

Inventors

• Fener CHEN · Shanghai, CN
• Zedu Huang · Shanghai, CN
• Ge Meng · Shanghai, CN
• Minjie LIU · Shanghai, CN
• Zhining Li · Shanghai, CN
• Zexu Wang · Shanghai, CN
• Haihui Peng · Shanghai, CN
• Fangjun XIONG · Shanghai, CN
• Yan Wu · Shanghai, CN
• Yuan Tao · Shanghai, CN

Key dates

• Filing date: Jan 14, 2018
• Grant date: Jan 7, 2020
• Expiry date: Apr 2, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/025
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure relates to the technical field of biochemical engineering and particularly discloses a preparation method for (R)-3-hydroxyl-5-hexenoate. In the method of the present disclosure, the (R)-3-hydroxyl-5-hexenoate is prepared by catalytic reduction of 3-carbonyl-5-hexenoate by ketoreductase with 3-carbonyl-5-hexenoate as the substrate. The amino acid sequence of ketoreductase is shown in SEQ ID NO.1. In the present disclosure, the (R)-3-hydroxyl-5-hexenoate having a very high chiral purity is obtained by asymmetric reduction by ketoreductase as the biocatalyst. The present disclosure has the advantages of easy operation, mild reaction conditions, high reaction yield and good practical industrial application value.