Cell line for screening influenza virus polymerase assembly inhibitor and construction method thereof
US10526634B2 · kind B2 · utility
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Key dates
| Filing date | Dec 28, 2016 |
| Grant date | Jan 7, 2020 |
| Priority date | — |
| Expiry date | Dec 28, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/11
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.