Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information

US10526649B2 · kind B2 · utility

Assignees

• Massachusetts Institute of Technology · US
• President and Fellows of Harvard College · US

Inventors

• Fei Chen · Thạnh Trung, VN
• Shahar Alon · Cambridge, US
• Andrew Payne · Cambridge, US
• Asmamaw Wassie · Boston, US
• Daniel E. Goodwin · Katy, US
• Edward S. Boyden · Sharon, US
• Evan R. Daugharthy · Cambridge, US
• Jonathan Scheiman · New York, US

Key dates

• Filing date: Oct 20, 2017
• Grant date: Jan 7, 2020
• Expiry date: Mar 2, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6841
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids in a process referred to herein as “expansion sequencing” (ExSEQ).