Method of detecting a microorganism in a sample by a fluorescence based detection method
US10526663B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 20, 2015 |
| Grant date | Jan 7, 2020 |
| Priority date | — |
| Expiry date | Feb 20, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/06113
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of detecting a microorganism, in particular Staphylococcus aureus, in a sample, comprising the steps of a) incubating the sample with a slow off-rate modified aptamer (SOMAmer) comprising a fluorescent label, b) optionally washing the sample, c) analyzing the sample by a fluorescence-based detection method.The invention further relates to a slow off-rate modified aptamer comprising a fluorescent label, wherein the SOMAmer comprises a nucleotide sequence specific for Staphylococcus aureus, its use for detecting Staphylococcus aureus cells in a sample, and to a microarray or biosensor and a kit comprising at least one of such SOMAmers comprising a fluorescent label.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.