Freeze-dried powder of high molecular weight silk fibroin, preparation method therefor and use thereof
US10533037B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 25, 2015 |
| Grant date | Jan 14, 2020 |
| Priority date | — |
| Expiry date | Mar 25, 2035 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61J3/02
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention discloses a freeze-dried powder of high molecular weight silk fibroin, preparation process and use thereof. The freeze-dried powder is obtained from silk by degumming, dissolution, dialysis, centrifugation, high temperature and high pressure treatment and freeze-drying. A method of preparing silk fibroin microspheres with polyethylene glycol comprises mixing a silk fibroin solution of 1-30 wt % with a PEG solution of 10-60 w % and incubating the resulting solution. A controlled-release or sustained-release silk fibroin gel formulation includes a gel-state carrier and a drug dispersed/adsorbed therein, the carrier is a silk fibroin gel formed by blending with LMW-PEG. The freeze-dried powder will not change the native molecular weight and random coil structure of silk fibroin, and can be rapidly dissolved into a solution, and can be stored and transported for a long time, and this reduces waste of silk fibroin materials and promotes R & D and biomedical applications of silk fibroin biomaterials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.