Cultured cell sheet, production method thereof, and application method thereof
US10533158B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 28, 2006 |
| Grant date | Jan 14, 2020 |
| Priority date | — |
| Expiry date | Nov 10, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2539/10
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.