Highly specific delivery of polynucleotides to the cell nucleus via compression
US10533189B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Sep 19, 2017 |
| Grant date | Jan 14, 2020 |
| Priority date | — |
| Expiry date | Mar 8, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/32
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The subject invention pertains to delivering a polynucleotide into a target cell, particularly, into the nucleus of the target cell. The method comprises the steps of contacting the polynucleotide with the cell and applying pressure on the polynucleotide and the cell in a manner that forces the polynucleotide into the cell. The pressure can be between about 0.1 Pa to about 50 Pa; whereas, the polynucleotide is contacted with the cell at a concentration of between about 0.1 μM and about 100 μM. The method can be practiced for cells in culture or cells in vivo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.