Artificial bioluminescent enzyme
US10533231B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 18, 2013 |
| Grant date | Jan 14, 2020 |
| Priority date | — |
| Expiry date | Oct 12, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y113/12007
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to establishment of a series of artificial luciferases based on artificial amino acid sequences extracted by amino acid alignment of copepod-derived luciferase sequences in a database based on amino acid similarity. The invention provides high luminescence intensity, high luminescence stability, and a spectrum with increased wavelength as luminescence characteristics. A series of artificial luciferases (ALuc) was established. The group of ALucs has superior luminescence characteristics, such as an increase in luminescence intensity, an increase in luminescence stability, or an increase in wavelength of the luminescence spectrum, which were not obtained before. Further, by using the artificial luciferases (ALuc) of the invention, it is possible to provide a novel, superior bioassay system, such as a bioluminescent probe, two-hybrid assay, a luminescent capsule, or the like having improved measurement function.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.