Production of red blood cells and platelets from stem cells

US10544393B2 · kind B2 · utility

Assignee

• BOSTON MEDICAL CENTER CORPORATION · US

Inventors

• George J. Murphy · Boston, US
• David H. Sherr · Dedham, US
• Sarah S. Rozelle · Brookline, US
• Brenden W. Smith · East Greenwich, US

Key dates

• Filing date: Jun 2, 2015
• Grant date: Jan 28, 2020
• Expiry date: Oct 4, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/10
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a stem cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR agonist are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided. In some embodiments the AhR modulator is an AhR antagonist. This disclosure also provides compositions comprising at least 1 million MEPs per ml and compositions in which at least 50% of the cells are MEPs.