Patent · US Active

Modified transposases for improved insertion sequence bias and increased DNA input tolerance

US10544403B2 · kind B2 · utility

70Cited by

6References

19Claims

0Family size

Assignee

Illumina, Inc. · US

Inventors

Christian Gloeckner · Bonn, DE
Amirali Kia · San Mateo, US
Erin Bomati · Santee, US
Molly He · San Diego, US
Haiying Li Grunenwald · Belleville, US
Scott Kuersten · Madison, US
Trina Faye Osothprarop · San Diego, US
Darin Haskins · Madison, US
Joshua Burgess · Sun Prairie, US
Anupama Khanna · Madison, US
Daniel Schlingman · Madison, US
Ramesh Vaidyanathan · Madison, US

Key dates

Filing date	Apr 29, 2019
Grant date	Jan 28, 2020
Priority date	—
Expiry date	Apr 29, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.