Patent · US Active

Bis-biotinylation tags

US10544449B2 · kind B2 · utility

26Cited by

23References

8Claims

0Family size

Assignee

PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

Gene Shen · Santa Clara, US
Natasha Popovich · Menlo Park, US
Erik Miller · Seattle, US
Satwik Kamtekar · Mountain View, US
Keith Bjornson · Fremont, US
Jeremiah Hanes · Woodside, US
Stephen Yue · Eugene, US
Lubomir Sebo · Redwood City, US
Zhong Wang · Zhejiang, CN

Key dates

Filing date	Jan 6, 2017
Grant date	Jan 28, 2020
Priority date	—
Expiry date	May 29, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC08G2650/04
WIPO fieldOrganic fine chemistry
WIPO sectorChemistry

Abstract

Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.