Sequencing methods, compositions and systems using terminator nucleotides

US10544455B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Wolfgang Hinz · Killingworth, US
• Peter Vander Horn · Foster City, US
• Earl Hubbell · Mountain View, US
• Christian Woehler · Darmstadt, DE

Key dates

• Filing date: Sep 16, 2015
• Grant date: Jan 28, 2020
• Expiry date: Nov 12, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.