Systems and methods for universal tail-based indexing strategies for amplicon sequencing

US10550427B2 · kind B2 · utility

Assignees

• The Translational Genomics Research Institute · US
• Arizona Board of Regents on Behalf of Northern Arizona University · US

Inventors

• James M. Schupp · Flagstaff, US
• Rebecca E. Colman · Flagstaff, US
• David Engelthaler · Flagstaff, US
• John D. Gillece · Phoenix, US
• Nathan Hicks · Phoenix, US
• Paul Keim · Flagstaff, US

Key dates

• Filing date: Nov 10, 2014
• Grant date: Feb 4, 2020
• Expiry date: Jan 8, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Some embodiments of the invention include a method of preparing a sample for sequencing that includes receiving a sample and amplifying at least one marker within the sample. In some embodiments, amplification of the first marker may include mixing the sample with a first oligonucleotide that comprises a first universal tail sequence and a second oligonucleotide that comprises a second universal tail sequence. In some aspects of the invention, the first universal tail sequence and the second universal tail sequence are different sequences.