Methods and systems for processing polynucleotides

US10550429B2 · kind B2 · utility

Assignee

• 10x Genomics, Inc. · US

Inventors

• Josephine Harada · San Francisco, US
• Tarjei Sigurd Mikkelsen · Dublin, US
• Katherine Pfeiffer · Berkeley, US
• Serge Saxonov · Oakland, US
• John R. Stuelpnagel · Lompoc, US

Key dates

• Filing date: May 30, 2019
• Grant date: Feb 4, 2020
• Expiry date: May 30, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/179
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.