System and method of data-dependent acquisition by mass spectrometry
US10553412B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 24, 2010 |
| Grant date | Feb 4, 2020 |
| Priority date | — |
| Expiry date | May 24, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6878
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems, computer-readable media, and methods using mass spectrometry to analyze a sample are provided. For example, a method includes: acquiring a precursor ion spectrum; analyzing the precursor ion spectrum to identify precursor ions that preliminarily match one or more peptides that each belong to at least one protein of interest for the analysis; selecting each of the identified precursor ions in an order according to a ranking protocol for maximizing the number of proteins that are identified in the sample; for each selected precursor ion: acquiring a corresponding product ion spectrum, determining whether the acquired product ion spectrum matches one of the peptides that belong to the set of proteins of interest, and identifying a matched peptide as being present in the sample; and identifying proteins of interest that are present in the sample based on the peptides that are identified as being present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.