Methods and compositions for improving plant traits

US10556839B2 · kind B2 · utility

Assignee

• Pivot Bio, Inc. · US

Inventors

• Karsten Temme · Berkeley, US
• Alvin Tamsir · San Francisco, US
• Sarah Bloch · Berkeley, US
• Rosemary Clark · Emeryville, US
• Emily Tung · Millbrae, US

Key dates

• Filing date: Apr 16, 2018
• Grant date: Feb 11, 2020
• Expiry date: Apr 16, 2038

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.