Method for detecting trace fungi using single-cell sequencing and kit thereof

US10557176B2 · kind B2 · utility

Assignee

• BEIJING INSTITUTE OF RADIATION MEDICINE · CN

Inventors

• Ying Yang · Nanhu, CN
• Shengqi WANG · Beijing, CN
• Zhe Zhou · Beijing, CN
• Min Hao Chen · Milpitas, US
• Peng Wang · Longbeilingcun, CN
• Zhen Li · Beijing, CN
• Zongwei LI · Beijing, CN

Key dates

• Filing date: Aug 5, 2016
• Grant date: Feb 11, 2020
• Expiry date: Aug 5, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/645
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for detecting trace fungi using single-cell sequencing and a fungi detection kit prepared by the method are disclosed. The method includes the steps of obtaining trace fungal cells, extracting fungal protoplasm by breaking the cell walls of fungi, extracting gDNA from trace fungal protoplasm and amplifying the gDNA, constructing trace gDNA library, genome sequencing, bioinformatics analysis and comparison, and determining the species of detected fungi, etc. The method realizes the high-efficiency detection of trace fungi, and can be directly applied to the isolation, detection, identification of trace and difficult-to-identify fungal samples or mixed samples, and in-depth study of genetic information. The fungi detection method and the kit are applicable to industrial production, environmental monitoring, air testing, soil testing, water quality testing, food testing, drug testing, cosmetics testing, health care products testing and medical testing, and other fields.