Probes for identifying geographical distribution and molecular epidemiology of viral hemorrhagic septicemia virus (VHSV) and uses thereof
US10557178B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 11, 2016 |
| Grant date | Feb 11, 2020 |
| Priority date | — |
| Expiry date | Aug 11, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a single-nucleotide polymorphism (SNP) for determining genotypes specific to the regions from which VHSV has been isolated, to PNA for identifying same and a method for identifying, by using same, the single-nucleotide polymorphism (SNP) for determining genotypes specific to the regions from which VHSV has been isolated and, more specifically, to PNA and a kit capable of detecting the single-nucleotide polymorphism mutations of C755A and A756G of the VHSV G-protein by using PNA comprising a sequence of SEQ ID NO: 1. The present invention enables an easy, rapid and accurate identification of genotypes specific to the regions from which VHSV has been isolated, by using PNA having an excellent binding affinity to DNA so as to make each genotype exhibit a different melting temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.