Signaling conjugates and methods of use

US10557851B2 · kind B2 · utility

Assignee

• Ventana Medical Systems, Inc. · US

Inventors

• Nelson Alexander · Marana, US
• William Day · Indianapolis, US
• Jerome W. Kosmeder · Tucson, US
• Mark Lefever · Tucson, US
• Larry E. Morrison · Glen Ellyn, US
• Anne M. Pedata · Tucson, US
• Stacey Stanislaw · Tucson, US

Key dates

• Filing date: Jul 18, 2018
• Grant date: Feb 11, 2020
• Expiry date: Jul 18, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/542
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.