Preparation of therapeutic exosomes using membrane proteins

US10561740B2 · kind B2 · utility

Inventors

• Kevin P. DOOLEY · New York, US
• Rane A. Harrison · Belmont, US
• Russell E. MCCONNELL · Watertown, US
• Ke Xu · Sudbury, US
• Damian J. Houde · Plymouth, US
• Nikki Ross · Cambridge, US
• Sonya Haupt · Cambridge, US
• John KULMAN · Belmont, US
• Douglas E. Williams · Redmond, US

Key dates

• Filing date: Dec 21, 2018
• Grant date: Feb 18, 2020
• Expiry date: Dec 21, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/60
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to methods of preparing a therapeutic exosome using a protein newly-identified to be enriched on the surface of exosomes. Specifically, the present invention provides methods of using the proteins for affinity purification of exosomes. It also provides methods of localizing a therapeutic peptide on exosomes, and targeting exosomes to a specific organ, tissue or cell by using the proteins. The methods involve generation of surface-engineered exosomes that include one or more of the exosome proteins at higher density, or a variant or a fragment of the exosome protein.