Method for significantly increasing lentiviral production

US10562939B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Stephen P. Goff · Orefield, US
• Yiping Zhu · New York, US
• Liang Tong · Chesterfield, US
• Shukun Luo · New York, US

Key dates

• Filing date: May 31, 2017
• Grant date: Feb 18, 2020
• Expiry date: May 31, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16222
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Increased viral particle maturation and production can be achieved in various methods for producing viral particles from viral proteins, in general, by inhibiting or preventing Heme Oxygenase 2 (HO-2) from binding to the group-specific antigen (Gag) of the viral proteins, thus allowing delivery of the viral proteins to plasma membranes where they can replicate and mature without interference from HO-2. The increase in viral particle maturation and production can also be achieved by minimizing or eliminating the presence of HO-2 to thus reduce or prevent binding of HO-2 to the group-specific antigen (Gag) of the viral proteins. The invention is particularly applicable to the production of lentiviruses from viral proteins wherein the Matrix domain (MA) of the Gag is myristoylated.