Base sequence for protein expression and method for producing protein using same
US10563209B2 · kind B2 · utility
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Key dates
| Filing date | Jul 15, 2016 |
| Grant date | Feb 18, 2020 |
| Priority date | — |
| Expiry date | Jul 15, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.
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