Genome engineering

US10563225B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• George M. Church · Brookline, US
• Luhan Yang · Somerville, US
• Marc Guell · Boston, US
• Joyce Lichi Yang · Cambridge, US

Key dates

• Filing date: Jun 30, 2014
• Grant date: Feb 18, 2020
• Expiry date: Jun 30, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods are provided for the use of Cas9 in genome engineering of stem cells. Methods include introducing into the stem cell a first foreign nucleic acid encoding a guide RNA complementary to a target DNA and which guides a Cas9 enzyme to the target DNA, wherein the RNA and the enzyme are members of a co-localization complex for the target DNA, introducing into the stem cell a second foreign nucleic acid encoding a donor nucleic acid sequence, wherein the guide RNA and the donor nucleic acid sequences are expressed, wherein the guide RNA and the Cas 9 enzyme co-localize to the target DNA, the Cas 9 enzyme cleaves the target DNA and the donor nucleic acid is inserted into the target DNA to produce altered DNA in the stem cell.