Patent · US Active

In situ nucleic acid sequencing of expanded biological samples

US10563257B2 · kind B2 · utility

12Cited by

2References

7Claims

0Family size

Assignees

Inventors

Edward S. Boyden · Sharon, US
Fei Chen · Thạnh Trung, VN
Shahar Alon · Cambridge, US
George M. Church · Brookline, US
Paul Warren Tillberg · Cambridge, US
Adam Marblestone · Arlington, US
Evan R. Daugharthy · Cambridge, US

Key dates

Filing date	Aug 23, 2018
Grant date	Feb 18, 2020
Priority date	—
Expiry date	Aug 23, 2038

Classification

Technology area (CPC B)Performing Operations; Transporting
CPC primaryB01L2300/043
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.