Prefixation for increased rare cell recovery
US10564075B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 23, 2016 |
| Grant date | Feb 18, 2020 |
| Priority date | — |
| Expiry date | Feb 23, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4088
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Traditionally, enriching or isolating rare circulating cells from a sample has proven difficult as rare circulating cells can be heterogeneous, which limits the use of techniques based on biological properties (e.g., cell surface markers), and can be flexible, which hinders techniques based on physical properties (e.g., diameter size). Methods disclosed herein are capable of enriching or isolating rare circulating cells are minimally impacted by heterogeneity or flexibility of rare circulating cells, and therefore, overcome disadvantages observed with prior techniques. For example, a method for enriching or isolating rare circulating cells from a sample of a subject including fixing one or more rare circulating cells in a sample to create a fixed sample, and isolating the one or more rare circulating cell from the fixed sample using a size exclusion technique is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.