Patent · US Active

Antibody-secreting cell assay

US10564160B2 · kind B2 · utility

0Cited by

2References

24Claims

0Family size

Assignee

MABTECH AB · SE

Inventors

Pia Dosenovic · Stockholm, SE
Gunilla Karlsson Hedestam · Stockholm, SE
Richard Wyatt · Village of La Jolla, US
Staffan Paulie · Nacka, SE

Key dates

Filing date	Aug 28, 2009
Grant date	Feb 18, 2020
Priority date	—
Expiry date	Mar 19, 2030

Classification

Technology area (CPC G)Physics
CPC primaryG01N2800/24
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.