Methods of introducing nucleic acids into cellular DNA
US10570400B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Feb 7, 2017 |
| Grant date | Feb 25, 2020 |
| Priority date | — |
| Expiry date | Aug 20, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.