Compositions and methods for detecting nicking enzyme and polymerase activity using a substrate molecule
US10570441B2 · kind B2 · utility
1Cited by
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20Claims
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Key dates
| Filing date | Jan 25, 2016 |
| Grant date | Feb 25, 2020 |
| Priority date | — |
| Expiry date | Aug 20, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions and methods for assaying the activity of nicking enzyme and polymerase in a reaction involving the use of a nucleic acid substrate molecule that detects nicking enzyme and polymerase extension activities by the release of a detectable reporter (e.g., a fluorophore).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.