Patent · US Active

Methods and systems for fluorescence detection

US10571396B2 · kind B2 · utility

2Cited by

5References

23Claims

0Family size

Assignee

Molecular Devices, LLC · US

Inventors

Annegret Schramm · Piding, DE
Michael Katzlinger · Eugendorf, AT

Key dates

Filing date	Feb 25, 2016
Grant date	Feb 25, 2020
Priority date	—
Expiry date	Mar 20, 2038

Classification

Technology area (CPC G)Physics
CPC primaryG01N2021/6441
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention is directed to multiplexed fluorescence detection, including time-resolved fluorescence (TRF) detection. A combination of spectral and temporal differences in fluorescence emission and spectral differences in excitation is used to enhance the ability to separate signals in an assay from multiple fluorescent labels. Different classes of labels may be utilized, including upconversion phosphors as well as lanthanide chelates and transition metal chelates. The methods may be implemented in optical plate readers, including cartridge-based multi-mode readers.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.