Plasmonic nanoparticle immunoassay method

US10571400B2 · kind B2 · utility

Assignee

• The General Hospital Corporation · US

Inventors

• Conor L. Evans · Boston, US
• W. Peter Hansen · Bridgewater, US
• Robert A. Hoffman · Mansfield, US
• Petra B. Krauledat · Canaan, US
• Daniel Cramer · Sharon, US

Key dates

• Filing date: Mar 11, 2016
• Grant date: Feb 25, 2020
• Expiry date: Jun 27, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/4725
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.