Method of manufacturing recombinant lectin protein and recombinant lectin protein manufactured by using the same
US10577400B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 30, 2018 |
| Grant date | Mar 3, 2020 |
| Priority date | — |
| Expiry date | Apr 30, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/405
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method of manufacturing a recombinant BPL3 protein comprising: synthesizing a recombinant BPL3 (rBPL3) gene encoding a Bryopsis plumosa lectin (BPL3) protein; preparing a tandem repeat rBPL3 gene including a repeating structure of the rBPL3 gene by tandemly binding the rBPL3 genes through spacers; preparing a recombinant plasmid by inserting the tandem repeat rBPL3 gene into an expression vector; and transforming an expression host by the recombinant plasmid. According to the method, it is possible to maximize expression efficiency of the recombinant lectin and enhance the activity of the manufactured recombinant lectin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.