Multi-photon counting for high sensitivity flow cytometer systems and methods for using the same
US10578542B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 19, 2018 |
| Grant date | Mar 3, 2020 |
| Priority date | — |
| Expiry date | Sep 19, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1486
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Aspects of the present disclosure include methods and systems for detecting light from a sample in a flow stream by multi-photon counting. Methods according to certain embodiments include irradiating a sample in a flow stream with a light source and detecting light from the sample in the flow stream and counting photons of the detected light by integrating photo-electron charge over a time interval. Methods also include irradiating a sample in a flow stream with a light source, detecting light from the sample in the flow stream and outputting a digital output signal and an analog output signal produced by the detected light. Systems for detecting light from a sample in a flow stream with a detector and counting photons by integrating photo-electron charge over a time interval are also described. Kits having a detector, a photon counter and a flow cell configured to propagate a sample in flow stream are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.