Method and device for generating a microscopy panoramic representation

US10582126B2 · kind B2 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Christian Muenzenmayer · Langenzenn, DE
• Alexander Gryanik · Erlangen, DE
• Tobias Bergen · Erlangen, DE
• Thomas Wittenberg · Erlangen, DE

Key dates

• Filing date: Oct 14, 2016
• Grant date: Mar 3, 2020
• Expiry date: Oct 14, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/20112
• WIPO field: Audio-visual technology
• WIPO sector: Electrical engineering

Abstract

Embodiments of the present invention relate to a method for generating a microscopy representation of a three-dimensional sample having a lateral extension in the x and y directions. The method has the following steps: a) arranging the flat three-dimensional sample on a positioner; b) recording the sample by means of an imaging device to obtain a first microscopy picture of the sample having a first section; c) altering the perspective onto the two-dimensional sample in the z direction; d) recording the sample by means of the imaging device to obtain a second microscopy picture of the sample having a second section; e) determining change information which allows drawing conclusions as to the change in perspective in the z direction, using a difference between the first and second microscopy pictures; and f) merging the first and second microscopy pictures while considering the change information to obtain the microscopy panoramic representation.