Methods for transfecting nucleic acid into live cells
US10583204B2 · kind B2 · utility
0Cited by
7References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 21, 2012 |
| Grant date | Mar 10, 2020 |
| Priority date | — |
| Expiry date | Mar 21, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.