Renilla based biosensors for monitoring biomolecule localization and trafficking in cells
US10584159B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 21, 2015 |
| Grant date | Mar 10, 2020 |
| Priority date | — |
| Expiry date | Feb 21, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/60
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Bioluminescence resonance energy transfer (BRET) biosensors for assessing the intracellular localization, internalization and trafficking into cellular compartments of proteins such as receptors, and other biomolecules such as second messengers, are disclosed. These biosensors, which are dependent on the concentration/density of the BRET donor and acceptor in cellular compartments rather that specific protein-protein interactions, use a Renilla GFP/Luc BRET pair, which allows the robust and reproducible monitoring of protein trafficking/localization, with a sensitivity compatible with high-throughput screening (HTS). The use of these biosensors for various applications, including assessing/monitoring protein endocytosis, recycling and intracellular trafficking, receptor maturation/rescue by pharmacological chaperones, various endocytosis/exocytosis processes, activation/inhibition, as well as biomolecule concentration/density in different cellular compartments, is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.